Characterization of Anaplasma ovis strains using the major surface protein 1a repeat sequences
نویسندگان
چکیده
منابع مشابه
Functional and Immunological Relevance of Anaplasma marginale Major Surface Protein 1a Sequence and Structural Analysis
Bovine anaplasmosis is caused by cattle infection with the tick-borne bacterium, Anaplasma marginale. The major surface protein 1a (MSP1a) has been used as a genetic marker for identifying A. marginale strains based on N-terminal tandem repeats and a 5'-UTR microsatellite located in the msp1a gene. The MSP1a tandem repeats contain immune relevant elements and functional domains that bind to bov...
متن کاملFunctional Epitope Core Motif of the Anaplasma marginale Major Surface Protein 1a and Its Incorporation onto Bioelectrodes for Antibody Detection
Anaplasmosis, a persistent intraerythrocytic infection of cattle by Anaplasma marginale, causes severe anemia and a higher rate of abortion, resulting in significant loss to both dairy and beef industries. Clinical diagnosis is based on symptoms and confirmatory laboratory tests are required. Currently, all the diagnostic assays have been developed with whole antigens with indirect ELISA based ...
متن کاملDiscrimination Between Anaplasma marginale and Anaplasma ovis by PCR-RFLP
Several tick-borne diseases with different etiological agents such as rickettsia, protozoa, bacteria and viruses can infect domestic ruminants. The only common feature between these diseases is that they can all be transmitted by ticks. Of these, Anaplasma spp. can cause diseases in the livestock with high economical losses. The Giemsa staining of blood smear is the common traditional method fo...
متن کاملConformational dependence of Anaplasma marginale major surface protein 5 surface-exposed B-cell epitopes.
The Anaplasma marginale outer membrane is composed of immunogenic major surface proteins (MSPs) linked both covalently and noncovalently in multimeric complexes (M. C. Vidotto, T. C. McGuire, T. F. McElwain, G. H. Palmer, and D. P. Knowles, Infect. Immun. 62:2940-2946). Consequently, effective induction of antibody against surface-exposed MSP epitopes has been postulated to require maintenance ...
متن کاملCharacterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale.
Major surface protein 5 (Msp5) of Anaplasma marginale is highly conserved in the genus Anaplasma and the antigen used in a commercially available competitive enzyme-linked immunosorbent assay (cELISA) for serologic identification of cattle with anaplasmosis. This study analyzes the degrees of conservation of Msp5 among various isolates of Anaplasma phagocytophilum and the extent of serologic cr...
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ژورنال
عنوان ژورنال: Parasites & Vectors
سال: 2017
ISSN: 1756-3305
DOI: 10.1186/s13071-017-2363-6